25P Prognostic classification of endometrial cancer according to transcriptomic-based immunophenotype

The Cancer Genome Atlas (TCGA) Project defined four prognostic subgroups of Endometrial (EC): POLE (favorable prognosis), MSI and Copy Number Low (CNL) the intermediate prognosis; High (CNH) unfavorable prognosis. aim study is to perform a characterization at immune level EC-TCGA groups identify those cases that could be benefited immunotherapy. A total 48 FFPE EC were retrospectively selected (Ref): (n=6); (n=9); CNL (n=16) CNH (n=17). Transcriptomic profiling was performed with HTG EdgeSeq Precision Immuno-Oncology Panel (PIO), which interrogates 1392 genes involved in tumor/immune interaction. estimation relative abundance stromal cellular content cell types assigned 23 Reveal software immunophenotyping signatures. DESeq2 R package used for differential expression analysis. Statistical analysis data visualization version 4.1.2. Clinicopathological information collected table. Unsupervised showed 2 clusters different prognosis terms OS (p=0.0033) DFS (p=0.00055). Cluster 1 constituted by 8 MSI, 6 POLE, 14 7 while cluster mainly formed (10), followed CNL. Differential resulted 897 between clusters, 400 overexpressed 497 under expressed 1. most significant TGFB1, BEX1, CDK4, TUBB, RFC4 TRIP13. In addition, related higher PD-1 (p=4.4·10-4), PD-L1(p=9.9·10-6) CTL4 (p=4·10-4) scores (immune; p=0.00019 stroma; p=0.00014).Table: 25PCluster 1Cluster 2TotalNo patients (%)35 (73)13 (27)48 (100)Histological type (%)Endometrial31 (65)9 (19)40 (84)Serous4 (8)4 (8)8 (16)Grade (%)117 (35)6 (13)23 (48)211 (23)3 (6)14 (29)37 (15)4 (8)11 (23)Stage (%)I25 (52)10 (21)35 (73)II1 (2)0 (0)1 (2)III9 (19)3 (6)12 (25)TCGA Group (%)POLE6 (12.5)0 (0)6 (12.5)MSI8 (16.7)1 (2.1)9 (18.8)CNL14 (29.2)2 (4.2)16 (33.3)CNH7 (14.6)7 (14.6)14 (29.2)Median follow-up [range] months89,9 [5-152]Median OS101,1 [26-152]85,33 [6-143]92,02 [6-152]Median DFS101,1 [10-152]81,17 [5-105]89,87 [5-152]Relapse (%)4/35 (11,4)7/13 (53,8)11/48 (22,9)Éxitus (%)1/35 (2,9)4/13 (30,8)5/48 (10,4) Open table new tab stratification suggests implication immune-related features classification EC-patients beyond TCGA subgroups. These findings useful clinical management disease, constituting an open window selection